Both actresses stand accused of using an illegal "side door" to get their children into college.
The actors will be among the first of the 50 affluent parents to face charges relating to the biggest college admissions scam ever uncovered in the US.
15 of the accused were summoned to Massachusetts on Wednesday, the state where prosecutors filed the criminal case.
Last month the scam's mastermind Rick Singer pled guilty to overseeing the $25million racket, which opened a "side door" to colleges for wealthy families, offering a number of cheating routes in for money, disguised as charitable donations.
"Fuller House" star Loughlin, along with her fashion designer husband Mossimo Giannulli, are accused of paying $500,000 to have their daughters Olivia Jade and Isabella Rose admitted to USC as members of the crew team, even though neither of them competitively rowed before.
"Desperate Housewives" star Huffman meanwhile is accused of paying $15,000 to have a proctor accomplice correct her daughter Sofia Grace's SAT scores before submitting them.
Though her husband William H Macy was allegedly aware of the scam, he has not been charged.
Both actresses were arrested in March and face felony charges of conspiracy to commit mail fraud and honest services mail fraud; they both could be sent to prison.
They appeared briefly in federal court in Los Angeles last month; Huffman was released on $250,000 bail, while Loughlin was released on a $1 million bond.
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